Formation of catecholaminergic neurons is affected in the ventral diencephalon of Zsur mutant embryos. A–J, Lateral views (A–F) (anterior is to the left, dorsal is to the top) and dorsal views (G–J) of th expression in the brain of wild-type (A, C, E, G,I) and Zsur mutant (B, D, F, H,J) embryos at 3 dpf. A–F, Overview of the catecholaminergic neurons (A, B), olfactory bulbs (C,D), and pretectum (E, F). In the ventral diencephalon of Zsur mutant embryos, the dopaminergic neurons are reduced in the posterior tuberculum (G,H) and hypothalamus (I, J). The LC (G, H) develops normally in Zsur mutants. vDD, Ventral diencephalic dopaminergic neurons; ObC, olfactory bulb catecholaminergic neurons; PrC, pretectal catecholaminergic neurons. Scale bars, 17 μm.