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. 2003 Jul 2;23(13):5919–5927. doi: 10.1523/JNEUROSCI.23-13-05919.2003

Figure 4.

Figure 4.

EGFR expression is modulated by Wnts and BMP4. A, Wnt 7a virus increases the proportion of cells that express a high level of EGFRs in E10.5 explants cultured for 5 d. Explants were dissociated and stained with anti-β-gal (D) to identify infected cells and anti-EGFR (E)to identify cells that express a high level of EGFRs. The effect of Wnt 7a on EGFR expression is inhibited by BMP4 (10 ng/ml). B, Wnt 7a and Wnt 7b also increase EGFR expression in explants of E11.5 cortex cultured for 5 d. C, Blocking Wnt signaling with mFz8-Fc (250 ng/ml per 2 explants) reduces the proportion of cells expressing a high level of EGFRs among control infected cells and cells infected with Wnt 7a virus. D, E, A Wnt 7a-infected cell expresses the viral marker β-gal (D, arrow) and EGFRs (E, arrow). *p ≤ 0.04 comparing Wnt with control virus, or mFz8-Fc with IgG-Fc; **p < 0.003 comparing Wnt 7a plus BMP with Wnt 7a.