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. 2003 Jul 2;23(13):5919–5927. doi: 10.1523/JNEUROSCI.23-13-05919.2003

Figure 6.

Figure 6.

Wnt stimulates proliferation independently of FGF2 or Shh. A, Signaling by exogenous or endogenous FGF2 was inhibited with FGF2-neutralizing antibodies in explants of E10.5 cortex for 4 d after infection with either control or Wnt 7a virus. Mouse or rabbit IgG was used for control. The proportion of infected (β-gal-positive) cells that were dividing (PCNA-positive) was determined immunocytochemically. Note that FGF2-neutralizing antibodies reduce proliferation stimulated by exogenous FGF2 (10 ng/ml) but not by Wnt 7a. B, Signaling by Shh in explants of E11.5 cortex was inhibited with either cyclopamine (0.5–5 μm) or a Shh-neutralizing antibody (5E1, 1:5 to 1:4) 4 d after infection with either control or Wnt 7a virus. DMSO was used for control. Proliferation was assessed as in A. *p ≤ 0.03 relative to control explants; **p ≤ 0.003 relative to control virus.