Figure 8.

P27 level is significantly decreased in the p75–ErbB1 ^KD mice. A, Quantification of p27 in Western blot analyses. Actin blot was used as a control. B, The spinal cord sections taken from P10 mice were stained for CC1 and p27 or CNPase and p27. Left, Low-magnification pictures; scale bar, 20μm. Middle and right, High-magnification pictures; scale bar, 8.3μm. Note that some cells in the p75–ErbB1 ^KD mice have p27 in the nucleus, but its level is much lower than that found in NTG (arrows). C, The p75–ErbB1 ^KD is expressed among some dividing oligodendrocyte progenitors. The p75–ErbB1 ^KD was detected by ErbB1 staining in the cerebellar white matter at P5 and P10. Note that the cells that are positive for ErbB1 contain BrdU immunoreactivity in their nucleus.