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. 2003 Jul 2;23(13):5407–5415. doi: 10.1523/JNEUROSCI.23-13-05407.2003

Figure 5.

Figure 5.

APP and FE65 colocalize with synaptophysin at synapses. a–c, Confocal images of the axonal arbors of 7–8 DIV neurons double-labeled with APP (a) and synaptophysin (b) antibodies. Yellow in the overlay (c) represents colocalization of APP and synaptophysin. Arrows point at a few of the synaptic puncta that contain synaptophysin and APP. d, Thresholding and multiplication of the images in a and b yield only the overlapping signal. By comparison with the thresholded synaptophysin signal, this image was used to determine the fraction of synaptophysin-containing sites that also contain APP. e–g, Confocal images of the axonal arbors of neurons double-labeled with FE65 (e) and synaptophysin (f) antibodies. Yellow in the overlay (g) represents colocalization of FE65 and synaptophysin. Arrows point at a few of the synaptic puncta that contain synaptophysin and FE65. h, Thresholding and multiplication of the images in e and f yield only the overlapping signal. By comparison with the thresholded synaptophysin signal, this image was used to determine the fraction of synaptophysin-containing sites that also contain FE65.