Figure 2.

The MZ is stratified with superficial C–R cells and deeper-migrating interneurons. A, Tangential view of the MZ after immunohistochemical staining for the CR-50 reelin antibody. Some C–R cell processes are grouped along orthogonal axes forming a distinct rectilinear pattern. A coronal semithin section of an E15 cortex stained for CR-50 reelin (brown) and counterstained with toluidine blue (B) illustrates the stratification. Red arrowheads denote the deeper tangentially oriented migrating cells. Electron micrographs from tangential sections though the MZ demonstrate the large C–R cells (C) often with processes oriented orthogonal to each other. Smaller cells with migrating morphology (D) lie deep to the C–R cells. Scale bars (C, D), 10μm.