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. 2003 Jul 2;23(13):5835–5845. doi: 10.1523/JNEUROSCI.23-13-05835.2003

Figure 7.

Figure 7.

Effects of reserpine, pargyline, and l-DOPA on cytosolic catecholamine homeostasis in rat chromaffin cells. A, Cytosolic catecholamine concentrations measured by IPE in CV mode (left) and total catechols measured by amperometric mode (right) in cells exposed to 1 μm reserpine and 10 μm pargyline. Left, Reserpine-treated group represents cells incubated with the drug for 5–60 min. Cells were pretreated with pargyline for 30 min before measurements or the addition of reserpine, which was added for 15 min. After the treatments (37°C; 7% CO2), the media were replaced with saline B1 containing the same drugs. Measurements were performed at RT during 60 min after the treatments. The following is the number of points in cells: untreated, n = 22; reserpine-treated, n = 21; pargyline-treated, n = 16; pargyline and reserpine-treated, n = 28. Right, Reserpine was added at time 0. Combined data from three experiments are grouped into 20 min time bins with n = 13–24 for different time point. B, Cytosolic concentrations of catecholamines (left) and total catechols (right) after cell exposure to 100 μm l-DOPA. Left, Middle bar represents cytosolic catecholamine concentration in chromaffin cells incubated with l-DOPA for 1–1.5 hr; recordings were made in the presence of l-DOPA. The right bar represents the level of catecholamines in cells preincubated with l-DOPA for 1 hr and washed twice with l-DOPA-free medium (10–60 min after l-DOPA withdrawal). The following is the number of points in cells: untreated, n = 25; l-DOPA-treated, 24; cells pretreated with l-DOPA after l-DOPA withdrawal, 17. Right, Time course of changes in the level of total cytosolic catechols after l-DOPA withdrawal. Data from four independent experiments are grouped into 20 min time bins (n = 14–24 for different time points). Data shown as mean ± SEM. Shaded areas represent one SD from the mean catechol concentrations in untreated chromaffin cells. All time points in the right panels and those that have asterisks in the left panels are significantly different from untreated cells (p < 0.001; ANOVA test).