Figure 4.
Effect of amyloid proteins on γ-secretase inhibitor neurotoxicity. A, Effect of 24 hr incubation with γ-secretase inhibitor on the viability of cortical neurons (open bars; n = 9), cerebellar granule neurons (striped bars; n = 9), and the SH-SY5Y cell line (hatched bars; n = 9) in the presence of different concentrations of Aβ1–40. B, Effect of 24 hr incubation with γ-secretase inhibitor (10 μm) on the viability of cortical neurons (open bars; n = 9), cerebellar granule neurons (striped bars; n = 9), and the SH-SY5Y cell line (hatched bars; n = 9) in the presence of different Aβ size forms at a concentration of 1 nm in each case. C, Toxicity of the monoclonal antibody 3D6 (1 μg/ml, 24 hr) on cortical neurons. Toxicity was reversed by coincubation with Aβ1–40 (1 nm) but not Aβ25–35 (1 nm). Aβ peptides alone had no effect on cell survival. In each case, n = 4.