Table 1.
A list of primer pairs used in RT‐PCR to detect seven bee viruses in the larvae and pupae of Apis cerana cerana
| Primers | Sequence (5′‐3′) | Product size (bp) | Source or references |
|---|---|---|---|
| SBV‐F | GCTGAGGTAGGATATTTGCGT | 824 | Chen, Pettis, Collins, & Feldlaufer (2006) |
| SBV‐R | TCATCATCTTCACCATCCGA | ||
| KBV‐F | GATGAACGTCGACCTATTGA | 415 | Thu et al. (2016) |
| KBV‐R | TGTGGGTTGGCTATGAGTCA | ||
| ABPV‐F | TTATGTGTCCAGAGACTGTATCCA | 900 | Choe, Nguyen, Hyun et al. (2012) and Choe, Nguyen, Jin et al. (2012) |
| ABPV‐R | GCTCCTATTGCTCGGTTTTTCGGT | ||
| CBPV‐F | AGTTGTCATGGTTAACAGGATACGAG | 455 | Singh et al. (2010) |
| CBPV‐R | TCTAATCTTAGCACGAAAGCCGAG | ||
| BQCV‐F | TGGTCAGCTCCCACTACCTTAAAC | 700 | |
| BQCV‐R | GCAACAAGAAGAAACGTAAACCAC | ||
| IAPV‐F | GGTCCAAACCTCGAAATCAA | 840 | |
| IAPV‐R | TTGGTCCGGATGTTAATGGT | ||
| DWV‐F | CTCGTCATTTTGTCCCGACT | 423 | |
| DWV‐R | TGCAAAGATGCTGTCAAACC | ||
| CSBV‐F | ACCTTCATCCAGTATCAGAACCAT | 157 | Huang et al. (2017) |
| CSBV‐R | ATAACCACCCGTCCCAGAG | ||
| apis‐β‐actin‐F | AATTTTCATGGTGGATGGTGC | 181 | Chen, Higgins, & Feldlaufer (2005) |
| apis‐β‐actin‐R | AGGAATGGAAGCTTGCGGTA |
SBV, sacbrood virus; KBV, Kashmir bee virus; ABPV, acute bee paralysis virus; CBPV, chronic bee paralysis virus; BQCV, black queen cell virus; IAPV, Israeli acute paralysis virus; DWV, deformed wing virus; CSBV, Chinese sacbrood virus; and apis‐β‐actin primer.