Figure 2.

Determination of cytotoxicity of the formulated functional nanoceria using MTT assay. (A) More than 70% of A549 cells were dead within 24 h when incubated with the combination of drugs carrying nanoceria, whereas, only 40% toxicity was observed with single drug delivered. However, more than 90% cells were found to be dead as a result of combination therapy after 48 h of incubation. (B) On the other hand, minimal toxicity was observed when functional nanoceria was incubated with H9c2 cells (FR-), suggesting for targeted delivery of drugs specifically to the tumor. 1× PBS solution is used for the treatment of control cells. Average values of three measurements are depicted ± standard error.