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Detection of apoptotic and necrotic cell death by fluorescence microscopy using annexinV-FITC and Ethidium Homodimer III. (A) Healthy cells emitted almost no fluorescence representing early apoptotic cells. (B) Apoptotic cells emitted green fluorescence after treatment with FNC-Doxo, and (C) Necrotic cells emitted red fluorescence when treated with FNC with combination of drugs. (i), (ii) & (iii) represents corresponding bright field images.
