Fig. 2.

Association of MASPs with collectin-11. a-d Association of MASPs with collectin-11 by flow cytometry. C. albicans were incubated with or without collectin-11 (10 µg/ml), MASPs (0.5 µg/ml of each MASP and MAP-1) or a mixture of both, respectively. Bound MASPs were assessed by flow cytometry as described in Materials and Methods. The mean fluorescence intensity was used to assess MASP binding. Results are representative of at least three independent experiments. e Association of MASPs with collectin-11 in ELISA. ELISA wells coated with BSA (5 µg/ml), collectin-11 (5 µg/ml) or MBL (5 µg/ml) were incubated with MASP-1, MASP-2, MASP-3 or MAP-1 (1 µg/ml of each), respectively. Bound MASPs were detected as described in Materials and Methods. Results are expressed as means ± SE from triplicate experiments. Results are representative of at least three independent experiments. * p < 0.01 vs. control.