Fig. 2.

Divalent cations reduce ATP-induced permeabilization of MLN mononuclear cells. Cells obtained from MLN were removed and adjusted to 2 × 10⁵ cells/sample. Cells were preincubated with copper, cadmium or zinc for 5 min and 50 µM ATP for 10 min at 37°C. During the last 5 min of incubation EB was added and samples analyzed by flow cytometry. Data are representative of at least 3 individual experiments performed in triplicate. * p < 0.05, ** p < 0.001, *** p < 0.0001.