Fig. 9.

Peritoneal PMN from NOX2-deficient mice display increased apoptosis. Flow cytometry was used to determine the percentage of PMN undergoing apoptosis in the peritoneum 24 and 48 h after SIRS induction. Apoptosis was evaluated by annexin V staining for exposed phosphatidylserine and Hoechst staining. Percentage of live (a; annexin V-negative, Hoechst-negative) and apoptotic (b; annexin V-positive) peritoneal PMN. Means + SEM (n ≥ 10 per genotype per time point). * p < 0.05; ** p < 0.01; *** p < 0.001.