Fig. 4.

IFIT1 and IFIT2 affect HBV S promoter activity. a Huh7 cells were cotransfected with pRT-TK and HBV promoter report plasmid, pSp1, pSp2, pCp or pXp, together with pcDNA3, pIFIT1 or pIFIT2. pRL-TK was used as an internal control. Relative luciferase activity was measured by dual-luciferase assay 48 h after transfection. The promoter activity of cells transfected with pcDNA3 was set as 100. b Huh7 cells were first transfected with siRNAs as indicated, then transfected with HBV promoter report plasmid together with pRL-TK as the internal control the next day. Luciferase activity was measured 48 h after plasmid transfection. The relative luciferase activity in cells transfected with siControl was set as 100.