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. 2019 Jun 4;20(9):1270–1280. doi: 10.1080/15384047.2019.1617571

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Figure 6. — LINC00958 Regulated Cell Growth, Motility, and EMT through YBX2 in OSCC. (a) The silencing of YBX2 by shYBX2#1 or shYBX2#2 in Fadu cells and the overexpression of YBX2 by pcDNA3.1/YBX2 in SCC15 cells were confirmed by RT-qPCR analyses. (b–c) SCC15 cells were transfected with shNC+pcDNA3.1, shLINC00958#1+ pcDNA3.1, or shLINC00958#1+ pcDNA3.1/YBX2. Fadu cells were transfected with pcDNA3.1+ shNC, pcDNA3.1/LINC00958+ shNC, or pcDNA3.1/LINC00958+ shYBX2#1 for subsequent experiments. CCK-8 and colony formation results of SCC15 and Fadu cells in each group. (d) Caspase-activity of SCC15 and Fadu cells in each group was evaluated to assess cell apoptosis level. (e) Transwell migration results of SCC15 and Fadu cells in each group. (f–g) RT-qPCR and western blot analysis of E-cadherin and N-cadherin levels of SCC15 and Fadu cells in each group.

*P < .05; **P < .01; ***P < .01.