Fig. 4.

TREM-1 induced Ca²⁺ mobilization is dependent on PI3K and p38MAPK in monocytic cells. The CD14⁺ subpopulation of MUTZ-3 cells enriched by MACS (upper panel), CD14⁺ monocytes (middle panel) or PMN (lower panel) (each 10⁶ cells/ml) were loaded with the Ca²⁺-sensitive fluorogenic dye FLUO3-AM. Anti-TREM-1 (clone 6B1) or control mAb (clone 4C9) (both 10 µg/ml) and goat anti-mouse F(ab')₂ (20 µg/ml) were added as indicated and Ca²⁺ flux was monitored over time in the absence or presence of PI3K (LY294002) or p38MAPK inhibitor (SB203580). The results from 1 representative of 3 independent experiments are depicted.