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. 2013 Nov 1;6(3):284–292. doi: 10.1159/000354976

Fig. 2.

Fig. 2

LAIR-2-Fc binds MBL and C1q with high affinity. Binding of LAIR-2-HIS to immobilized MBL (a) or C1q (b) and binding of LAIR-2-Fc to immobilized MBL (c) or C1q (d) was measured by surface plasmon resonance analysis. Recombinant proteins were injected at a flow rate of 20 µl/min at 25°C through a BIAcore flow cell containing 1,100 RU C1q for the HIS and Fc fusion proteins, 900 RU MBL for the HIS proteins or 1,500 RU MBL for the Fc proteins. In the left panels, individual symbols representing resonance at equilibrium along with the corresponding concentration of the protein are shown. Triplicate binding curves of LAIR-2-HIS at a concentration of 0, 500, 1,000, 2,000, 3,000, 4,000, 5,000, 6,000, 8,000, 10,000, 12,000, 16,000, 20,000 and 24,000 nM and LAIR-2-Fc at a concentration of 0, 31.25, 62.5, 125, 250, 500, 1,000, 2,000, 3,000, 4,000, 6,000 and 8,000 nM are shown in the right panels. Values are means ± SEM.