Fig. 1.

S. pyogenes induces expression of TREM-1 in BMDM. a Expression of trem-1 mRNA on BMDM after infection with S. pyogenes at MOI of 1:1, 10:1 and 100:1 bacteria per macrophage. BMDM were exposed to S. pyogenes for 1 h, washed and further incubated in the presence of gentamicin for 4 h. Total RNA was isolated followed by RT-PCR analysis of trem-1 and β-actin gene expression. b Quantitative expression of trem-1 mRNA on BMDM after infection with S. pyogenes at MOI of 1:1, 10:1 and 100:1 bacteria per macrophage measured by real-time PCR. c Fluorescence microscope photographs of TREM-1 in BMDM infected with S. pyogenes at MOI of 1:1 (cii), 10:1 (ciii) and 100:1 (civ) bacteria per macrophage. TREM-1 expression on uninfected macrophages is also shown (ci). TREM-1 appears in red and S. pyogenes in green. Macrophage nuclei are stained by DAPI (blue). Bar = 25 µm.