Table 3.
Checkerboard analysis of monocyte migration in response to laminin LG4 peptides 3-SFM29, 4-TLF20, 5-DGR12, LL-37 and fMLP with indicated molar concentrations relative to the control set as 1
| Upper chamber (M) |
|||
|---|---|---|---|
| 0 | 10–7 | 10–5 | |
| Lower chamber (M) | |||
| 3-SFM29 | |||
| 0 | 1.0 ±0.3 | 4.8 ± 2.7 | 10.0± 2.4 |
| 10–7 | 1.1 ±1.5 | 0.2 ± 0.1 | 10.0± 4.0 |
| 10– 5 | 14.0 ±3.7 | 9.9 ± 1.2 | 7.8± 0.9 |
| 4-TLF20 | |||
| 0 | 1.0 ±0.3 | 0.4 ± 0.3 | 4.2± 1.4 |
| 10–7 | 3.5 ±1.3 | 1.1 ± 1.4 | 3.3± 1.0 |
| 10– 5 | 34.8 ±3.9 | 21.7 ± 2.3 | 11.9± 0.4 |
| 5-PGR12 | |||
| 0 | 1.0 ±0.5 | 5.2 ± 2.0 | 0.5± 0.02 |
| 10–7 | 28.6 ±2.6 | 13.6 ± 5.3 | 0.6± 0.1 |
| 10–5 | 1.7 ±0.1 | 1.1 ± 0.1 | 0.8± 0.3 |
| LL-37 | |||
| 0 | 1.0 ±0.2 | 2.8 ± 0.4 | 3.0± 0.8 |
| 10–7 | 2.0 ±0.7 | 0.4 ± 0.2 | 2.5± 0.9 |
| 10–5 | 10.0 ±1.3 | 6.5 ± 1.4 | 1.5± 0.4 |
| fMLP | |||
| 0 | 1.0 ±0.3 | 1.0 ± 0.6 | 3.7± 1.3 |
| 10– 9 | 0.4 ±0.5 | 1.4 ± 1.1 | 3.1± 2.3 |
| 10–8 | 17.4 ±5.1 | 15.5 ± 4.8 | 4.0± 2.0 |
The means ± SD of 3 independent experiments are shown. The concentrations in the upper chamber for fMLP are equal to those in the lower chamber: 0, 10–9 and 10–8 M, respectively.