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. 2019 Aug 29;55(4):879–895. doi: 10.3892/ijo.2019.4863

Figure 6.

Figure 6

JQ1 induces cycle arrest and apoptosis of glioma stem cells through the VEGF/PI3K/AKT signaling pathway. (A) The mRNA expression of genes related growth factors in CSC2078 expressed as fold change. (B and C) Western blotting analysis of VEGF, MMP9 and MMP2 expression in CSC2078 treated with JQ1 (error bars represent standard error of the mean). (D and E) CSC2078 cells were treated with JQ1 and or linifanib for 24 h. JQ1=100 nM, linifanib=5 µM. The cell cycle was analyzed by flow cytometry. Quantitation of the cell cycle. Data are representative of three independent experiments (error bars represent standard error of the mean). (F and G) CSC2078 cells were treated with JQ1 and or linifanib for 24 h. JQ1=100 nM, linifanib=5 µM. Effects of CSC2078 apoptosis were detected by flow cytometry. Quantitation of the apoptosis rate of CSC2078. Data are representative of three independent experiments. (Error bars represent standard error of the mean). (H and I) Western blotting analysis of p-VEGFR2, VEGFR2, PI3K, Akt, p-Akt (Ser473), CyclinB1, Bcl-2 and Bax in control, JQ1 and/or linifanib treated groups. JQ1=100 nM, linifanib=5 µM. Densitometry was performed and fold change of protein expression was presented (error bars represent standard error of the mean). *P<0.05, **P<0.01 vs. Con. Bax, Bcl2-associated X protein; Con, control; J/C, JQ1/Con; MMP, matrix metalloproteinase; p, phosphorylated; VEGFR2, vascular endothelial growth factor; VEGFR2, receptor 2.