Fig. 7.

Dopamine induces transient phosphorylation of neostriatal InsP₃R1 by PKA. a, Time course of changes in neostriatal InsP₃R1 PKA-phosphorylated state in response to application of 100 μm dopamine. Dopamine was applied to neostriatal slices at time 0. At each time point, the fraction of neostriatal InsP₃R1 in the PKA-phosphorylated state is shown as mean ± SE (n = 3) (filled circles). b, c, The same experiment as in a performed with slices exposed to 400 μm calyculin A (CalA, b) (open circles) or 10 nm okadaic acid (OA, c) (filled squares) for 60 min before the application of dopamine.