Fig. 2.

CGRP increased ¹²⁵I-SP binding to neonatal rat spinal neurons in a concentration-dependent manner. Ligand binding was measured after treatment with CGRP for 24 hr. IBMX (50 μm) was included in all of the treatments, and the average amount of specific binding in the presence of IBMX alone within an experiment was subtracted from all values for that experiment. Data were normalized to the average for control binding within each experiment. *Significantly different from the control group atp < 0.05 (1-way ANOVA with Tukey's multiple comparisons test). n = 11–24 wells per concentration (2–4 wells per concentration per experiment, 4–8 experiments per concentration).