Table 1.
The effect of CGRP on 125I-SP binding occurred predominately on neurons
| Type of culture | Treatment | 125I-SP binding (fmol/mg of protein) | Protein (μg/well) |
|---|---|---|---|
| Neuron-enriched | Control | 11.2 ± 1.2 (4)* | 56 ± 3 (4) |
| CGRP (1 μm) | 13.5 ± 1.8 (4)*,1-160 | ||
| Non-Neuronal | Control | 0.9 ± 0.4 (3) | 49 ± 10 (3) |
| CGRP (1 μm) | 1.5 ± 0.3 (3)1-160 |
Specific binding of 125I-SP was determined 24 hr after treatment. Numbers in parentheses are numbers of experiments.
*
Significantly different from same treatment of non-neuronal cells; p < 0.001 (two-way ANOVA with Tukey's multiple comparisons test).
F1-160: Significantly different, p < 0.05 (Student's t test, paired) relative to control within the same culture preparation.