Table 2.
CGRP receptors mediated the CGRP-induced increase of cAMP in neuron-enriched cultures
| Treatment | cAMP (pmol/mg protein) | Effect of CGRP8–37 on cAMP |
|---|---|---|
| Control | 19 ± 1 (30) | 23 ± 3 (15) |
| CGRP (1 × 100 nm) | 44 ± 4 (7) | 7 ± 1 (7) |
| CGRP (2 × 100 nm) | 112 ± 7 (12)* | 28 ± 2 (12) |
| CGRP (3 × 100 nm) | 230 ± 15 (7)* | 68 ± 2 (7)2-160 |
CGRP8–37 was used at 3 μm. Multiple treatments occurred at 2 hr intervals. Samples were collected 10 min after the last addition of agonist. Control groups for multiple addition of CGRP were not different from each other, so these groups were pooled. Numbers in parentheses are numbers of wells per treatment (two or three wells per treatment per experiment, three to five experiments per treatment).
*
p < 0.001 compared with control within the same group and cAMP production in the presence of antagonist.
F2-160: p < 0.05 compared with control within the same group (two-way ANOVA with Tukey's multiple-comparisons test).