Table 3.
Treatment with PGE2 did not increase125I-SP binding
| Treatment | cAMP (pmol/mg of protein) | 125I-SP binding (% of control) |
|---|---|---|
| Control | 17 ± 3 (20) | 100 ± 3 (17) |
| PGE2 (1 × 1 μm) | 72 ± 11 (9)3-150 | 98 ± 5 (10) |
| PGE2 (3 × 1 μm) | 147 ± 5 (9)3-150 | 84 ± 3 (6)3-160 |
Multiple treatments occurred at 2 hr intervals. Samples were collected 10 min after the last addition of PGE2 for determination of cAMP, and 125I-SP binding was determined 24 hr after initiation of treatment. 125I-SP binding was normalized to the mean of samples for control binding within the same culture preparation. Control groups for multiple additions of PGE2 were not different from each other so these groups were pooled. Numbers in parentheses are numbers of wells per treatment (two or three wells per treatment per experiment, three or four experiments per treatment with the exception of SP-binding after 3 × 1 μm PGE2, which was replicated once.
F3-150: p < 0.001 compared with all other groups within the measure.
F3-160: p < 0.05 compared with control within the measure (one-way ANOVA with Tukey's multiple-comparisons test).