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. 2003 Mar 1;23(5):1769–1780. doi: 10.1523/JNEUROSCI.23-05-01769.2003

Fig. 4.

Fig. 4.

Alterations in RMS, VZ, and granular layer of the OB in Mash1−/− embryos. A,B, D–K, Sagittal sections, 12 μm (A, B, F,I) and 20 μm (D,E, G, H, J,K), through the heads of E17.5Mash1+/+ embryos (A, D,F–H) and Mash1−/− littermates (B, E, I–K) were processed for BrdU or NeuN immunostaining or in situhybridization for 3′ Mash1 UTR, Gad67, orReelin as indicated. Scale bars: (in B)A, B, 200 μm; (inI) F, I, 100 μm; (in E) D, E,G, H, J, K, 200 μm. C, Quantification of BrdU-incorporating cells in the ventricular zone and granular layer of the OB in Mash1−/− embryos (gray bars) and wild-type (striped bars) littermates. The total number of BrdU+ cells was counted in a series of three 83-μm-wide (approximate width of the ventricular zone) bands proceeding dorsally or ventrally from the OB ventricular surface (inset). Values: Band I, 107 (range, ±11) BrdU+ cells (wild type) and 85.5 (range, ±10.5) BrdU+ cells (Mash1−/−); Band II, 103 (range, ±6) BrdU+ cells (wild type) and 2 (range, ±2) BrdU+ cells (Mash1−/−); Band III, 27 (range, ±2) BrdU+ cells (wild type) and 8.5 (range, +4.5) BrdU+ cells (Mash1−/−).