Table 3.
Kinetic parameters and inhibitor IC50 values for glutamate uptake in synaptosomes prepared from various brain regions of transgenic G93A rats and age-matched controls
| Kinetics | Pharmacology | |||
|---|---|---|---|---|
| Vmax (pmol · min−1 · mg−1) | Km(μm) | PDC (μm) | DHK (μm) | |
| Cortex | ||||
| CON | 669 ± 16 | 8 ± 1 | 2.1 ± 0.3 | 38.2 ± 0.5 |
| G93A | 603 ± 74 | 7 ± 0 | 2.0 ± 0.1 | 31.9 ± 7.2 |
| Hippocampus | ||||
| CON | 503 ± 39 | 5 ± 0 | 1.8 ± 0.1 | 35.7 ± 10.9 |
| G93A | 523 ± 96 | 6 ± 0 | 1.7 ± 0.2 | 35.1 ± 6.2 |
| Striatum | ||||
| CON | 422 ± 60 | 5 ± 0 | 1.7 ± 0.1 | 32.0 ± 6.1 |
| G93A | 378 ± 72 | 6 ± 0 | 1.8 ± 0.4 | 31.5 ± 5.0 |
| Cerebellum | ||||
| CON | 74 ± 8 | 6 ± 0 | 3.6 ± 0.3 | >1000 |
| G93A | 65 ± 5 | 5 ± 0 | 3.3 ± 0.3 | >1000 |
| Brainstem | ||||
| CON | 127 ± 20 | 6 ± 0 | 2.0 ± 0.2 | 87.6 ± 22.4 |
| G93A | 123 ± 29 | 6 ± 1 | 2.0 ± 0.1 | 79.3 ± 19.5 |
Glutamate uptake in synaptosome fractions prepared from the indicated brain regions of G93A and age-matched control (CON) rats was evaluated at end stage of disease progression. Kinetic parameters and IC50 values are mean ± SEM values from three independent experiments.