Skip to main content
. 2003 Mar 15;23(6):2193–2202. doi: 10.1523/JNEUROSCI.23-06-02193.2003

Fig. 8.

Fig. 8.

The effect of huntingtin context on the activation of caspases in transfected HEK293 cells. A, Western blot showing the expression of transfected huntingtin containing different protein sizes. The bracket indicates the stacking gel in which aggregated huntingtin is present.B, Immunofluorescence labeling of transfected HEK293 cells expressing 150Q-exon 1, 120Q-208, 120Q-508, and 120Q-945.C, Caspase-3 and caspase-9 activities of transfected cells that expressed different huntingtin fragments as indicated. The data were obtained from the 48 hr transfection of cells of three independent transfections and were presented as means ± SEM of control. The control is the activity of nontransfected cells.D, MTS assays of the viability of huntingtin-transfected cells. The control is the viability of nontransfected cells. The data (means ± SD) were obtained from three to four transfections for 72 hr. *p < 0.05 and **p < 0.01 compared with the activity of the vector-transfected cells.