Table 1.
c-Fos expression in mice after limited access to test solutions
| B6 Strain | D2 Strain | ANOVA | |||||
|---|---|---|---|---|---|---|---|
| Water | Sucrose | Ethanol/sucrose | Water | Sucrose | Ethanol/sucrose | ||
| Hippocampus | |||||||
| DG | 5.0 ± 1.1 | 6.1 ± 1.0 | 4.5 ± 0.8 | 8 ± 1.3 | 12.5 ± 7.2 | 6.2 ± 0.9 | |
| CA1 | 0.3 ± 0.1 | 1.1 ± 0.6 | 0.2 ± 0.1 | 0.3 ± 0.2 | 0.3 ± 0.2 | 0.3 ± 0.2 | |
| CA2 | 0 ± 0 | 0 ± 0 | 0.1 ± 0.1 | 0 ± 0 | 0.4 ± 0.2 | 0.4 ± 0.4 | |
| CA3 | 2.0 ± 0.7 | 1.5 ± 0.4 | 2.2 ± 1.0 | 1.3 ± 0.71-a | 3.7 ± 0.8 | 1.1 ± 0.41-a | GxS |
| Septum and striatum | |||||||
| AcbC | 0.1 ± 0.1 | 0.3 ± 0.2 | 0.1 ± 0.1 | 0.1 ± 0.1 | 0.4 ± 0.2 | 0.4 ± 0.2 | |
| AcbS | 0.9 ± 0.4 | 1.5 ± 0.3 | 1.3 ± 0.3 | 1.6 ± 0.2 | 1.9 ± 0.4 | 1.6 ± 0.3 | |
| BNST | 2.8 ± 0.5 | 3.4 ± 0.9 | 2.8 ± 0.6 | 3.3 ± 0.8 | 3.3 ± 0.9 | 2.0 ± 0.8 | |
| LSd | 2.0 ± 0.3 | 2.6 ± 0.5 | 1.4 ± 0.6 | 1.0 ± 0.3 | 2.7 ± 0.5 | 1.6 ± 0.4 | G |
| LSi | 1.9 ± 0.5 | 2.3 ± 0.3 | 1.0 ± 0.21-a | 0.9 ± 0.4 | 2.2 ± 0.41-b | 2.0 ± 0.31-b | G, GxS |
| LSv | 1.4 ± 0.2 | 1.7 ± 0.5 | 0.8 ± 0.3 | 1.3 ± 0.5 | 1.4 ± 0.4 | 1.5 ± 0.5 | |
| MS | 1.6 ± 0.4 | 2.0 ± 0.5 | 1.4 ± 0.4 | 1.3 ± 0.3 | 1.2 ± 0.4 | 0.5 ± 0.2 | S |
| Amygdala | |||||||
| La | 0 ± 0 | 0.1 ± 0.1 | 0.1 ± 0.1 | 0 ± 0 | 0 ± 0 | 0.4 ± 0.1 | |
| BLA | 1.0 ± 0.5 | 1.8 ± 0.5 | 1.7 ± 0.7 | 0.4 ± 0.2 | 1.6 ± 0.7 | 0.9 ± 0.4 | |
| CeM | 1.3 ± 0.6 | 1.1 ± 0.5 | 1.7 ± 0.7 | 1.7 ± 0.5 | 2.6 ± 0.6 | 2.7 ± 0.8 | |
| CeL | 0.7 ± 0.4 | 1.6 ± 0.6 | 2.0 ± 0.6 | 0.9 ± 0.5 | 1.2 ± 0.6 | 1.7 ± 0.7 | |
| Hypothalamus and thalamus | |||||||
| AHP | 6.8 ± 1.4 | 5.7 ± 1.4 | 5.8 ± 1.6 | 2.8 ± 1.7 | 7.3 ± 3.8 | 1.1 ± 0.5 | |
| LH | 1.8 ± 0.8 | 1.3 ± 0.5 | 0.6 ± 0.3 | 0.9 ± 0.3 | 2.9 ± 0.8 | 1.1 ± 0.8 | |
| Pa | 9.0 ± 4.3 | 4.8 ± 1.4 | 9.0 ± 2.8 | 2.8 ± 1.2 | 2.1 ± 0.6 | 3.7 ± 3.0 | S |
| PV | 13.7 ± 5.1 | 7.0 ± 1.2 | 12.0 ± 3.5 | 20.1 ± 5.3 | 35.7 ± 6.7 | 22.9 ± 5.8 | S1-160 |
| SO | 1.6 ± 0.4 | 0.9 ± 0.3 | 0.9 ± 0.3 | 1.7 ± 0.2 | 1.0 ± 0.4 | 1.7 ± 0.6 | |
| Neocortex | |||||||
| M | 0.4 ± 0.3 | 0.4 ± 0.2 | 0.2 ± 0.1 | 0.3 ± 0.2 | 0.1 ± 0.1 | 0.2 ± 0.2 | |
| Pir | 3.9 ± 0.6 | 5.3 ± 0.7 | 5.1 ± 1.3 | 5.4 ± 1.0 | 6.7 ± 1.2 | 3.7 ± 0.7 | |
| RS | 0.3 ± 0.2 | 0.2 ± 0.1 | 0 ± 0 | 0.1 ± 0.1 | 0.6 ± 0.2 | 1.5 ± 1.1 | |
| S1 | 0.3 ± 0.2 | 0.3 ± 0.2 | 0.3 ± 0.2 | 0.3 ± 0.2 | 0.4 ± 0.2 | 0.3 ± 0.2 | |
| Midbrain | |||||||
| EW | 6.8 ± 1.2 | 10.4 ± 1.7 | 17.9 ± 1.9 | 3.7 ± 1.0 | 4.8 ± 0.8 | 7.4 ± 3.2 | G1-160, S1-160 |
| VTA | 0.6 ± 0.2 | 0.4 ± 0.2 | 0.4 ± 0.2 | 0.6 ± 0.3 | 0.2 ± 0.1 | 0.3 ± 0.2 | |
Data are presented as mean ± SEM. AcbC, Core of nucleus accumbens (+1.1 mm); AcbS, shell of nucleus accumbens (+1.1 mm); AHP, posterior portion of anterior hypothalamus (−1.22 mm); BLA, basolateral amygdala (−1.2 mm); BNST, bed nucleus of stria terminalis (+0.5 mm); CA1 (−1.5 mm); CA2 (−1.5 mm); CA3 (−1.5 mm); CeL and CeM, capsular, lateral, and medial divisions of central nucleus of amygdala (−1.2 mm); DG, dentate gyrus (−1.5 mm); EW, Edinger-Westphal nucleus (−3.8 mm); LH, lateral hypothalamus (−0.9 mm); LSD, LSI, and LSV, lateral septum dorsal, intermediate, and ventral (+0.7 mm); M, motor cortex (−1.2 mm); MS, medial septum (+0.7 mm); Pa, paraventricular nucleus of hypothalamus (−0.9 mm); PH, posterior hypothalamus (−2.5 mm); Pir, piriform cortex (−1.2 mm); PV, paraventricular nucleus of thalamus (−0.9 mm); RS, retrosplenial agranular cortex (−1.2 mm); S1, somatosensory cortex (−1.2 mm); VTA, ventral tegmental area (−3.5 mm); ANOVA: G, significant effect of group; S, significant effect of strain; GxS, significant group by strain interaction, p< 0.05.
F1-160: p < 0.001.
Fisher's post hoccomparisons for brain areas with significant interactions. Significantly different from sucrose group of same strain.
Fisher's post hoccomparisons for brain areas with significant interactions. Significantly different from water group of same strain.