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. 2003 Mar 15;23(6):2371–2382. doi: 10.1523/JNEUROSCI.23-06-02371.2003

Fig. 4.

Fig. 4.

Role of cAMP in the effects of endocannabinoids on ERK phosphorylation. A, Rat hippocampal slices were incubated with forskolin (50 μm) for the indicated period of time. Homogenates were analyzed for active dually phosphorylated ERK by immunoblotting. B, Rat hippocampal slices were incubated as described in Materials and Methods, in the presence or in the absence of 4 mm 8-Br-cAMP for 45 min before the addition of vehicle (Control), 1 μmanandamide, or 2-AG for 5 min. In other experiments, slices were incubated in the presence of the PKA inhibitors H-89 (100 μm) or Rp-cAMPS (1 mm) for 20 min. Homogenates were analyzed for active dually phosphorylated ERK by immunoblotting as described in the legend to Figure 1.C, Quantification of the results for P-ERK2 as described in the legend to Figure 1. Data correspond to mean ± SEM. Statistical analysis was done with ANOVA (8-Br-cAMP:F(5,18) = 14.5; H89 and RpcAMPs:F(2,7) = 16.1; p < 0.01) followed by t test (treated vs control: ***p < 0.001, **p < 0.01, *p < 0.05; treated in the presence of 8-Br-cAMP vs in its absence: °° p < 0.01, ° p < 0.05).