Recombinant virus vector was constructed from an adeno-associated virus. With the exception of the terminal repeats (TR), all viral genes were removed, making the virus replication-defective, and were replaced with genes for NGF and/or GFP under the control of cytomegalovirus/chicken β-actin hybrid promoter (CB). A, pCB-NGF; B, pTR-UF12.1. IRES, Internal ribosome entry site.