Innervation is required for the maintenance of UNC-49 clusters. A, SNB-1–CFP expressed in the GABA nervous system of lin-6(e1466) L4 larvae. In this mutant, the GABAergic ventral motor neurons are absent, and no synaptic varicosities are observed along the ventral cord (closed arrowheads). The fluorescence in the middle of the animal corresponds to autofluorescent gut granules. B, Localization of UNC-49–YFP in the same individual, showing a disorganization of UNC-49–YFP expression pattern ventrally (closed arrowheads) while dorsally presynaptic and postsynaptic markers appear wild type (open arrowheads). Scale bar, 20 μm.