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. 2003 Apr 1;23(7):2591–2599. doi: 10.1523/JNEUROSCI.23-07-02591.2003

Fig. 4.

Fig. 4.

GABA receptors cluster at ectopic GABA release sites in unc-5 mutants. A, Localization of SNB-1–CFP expressed in the GABAergic system ofunc-5(e152) adult mutant. The migration of motor neuron axons to the dorsal cord is disrupted. Presynaptic varicosities are observed in ectopic axons located along the lateral side of the body (open arrows). The dashed line indicates the dorsal side of the worm. B, Localization of UNC-49–YFP expressed in the same individual. UNC-49–YFP is found along the ventral cord and at ectopic lateral positions (open arrows). Inset shows a high amplification of the UNC-49–YFP signal, which enables the visualization of ectopic muscle arms extending ventrally from dorsal muscles (arrowhead). C, Merging SNB-1–CFP and UNC-49–YFP signals demonstrates that lateral clusters of UNC-49–YFP are colocalized with ectopic presynaptic varicosities (inset as in B). Scale bar, 20 μm.