Layer 2/3 neuron MDFs are elongated along the axis of preferred orientation, whereas layer 4 neuron MDFs are radially symmetric. A, Histogram of MDF aspect ratios (long side/short side) for layer 2/3 neurons (black) and layer 4 neurons (white). In the third bin the counts for layers 2/3 and 4 were the same, represented by a hashed bar. B, MDF length versus width for layer 2/3 (black diamonds) and layer 4 (open circles), where length is measured along the axis of preferred orientation. C, Schematic of average MDF size and shape for layer 2/3 (top) and layer 4 (bottom) ± 1 SD (dashed lines).