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. 2003 Apr 1;23(7):2804–2814. doi: 10.1523/JNEUROSCI.23-07-02804.2003

Fig. 2.

Fig. 2.

Expression patterns of PTPζ and PTN in postnatally developing cerebellum. A–C, Confocal microscopic images of cerebellar cryosections derived from P9 rats and double stained by fluorescent immunohistochemistry using antibodies against the extracellular domain (ECD) (A1) or the intracellular domain (ICD) (B1, C1) of PTPζ (Cy2) and IP3R (A2, B2) or GLAST (C2) (Cy3). A3, B3, andC3 are the merged images. The immunoreactivities to PTPζ-ICD and GLAST partially overlapped each other, especially around Purkinje cells. EGL, External granular layer;ML, molecular layer; PL, Purkinje cell layer; IGL, internal granular layer. Scale bar, 25 μm.D, E, A cerebellar cryosection derived from a P9 rat and stained by immunohistochemistry using an antibody against PTN. E is the high-magnification image of the enclosed area in D. PTN distributes abundantly in the ML. Scale bars: D, 50 μm; E, 25 μm.F, An adjacent section stained with toluidine blue. Scale bar, 25 μm.