Fig. 6.

Effects of the Chase ABC and CS treatments on PTN contents in the cerebellum. A, B, Confocal microscopic images of adjacent cryosections derived from the cerebellum of a P9 rat and stained by fluorescent immunohistochemistry using an antibody against PTN without (A) or with (B) pretreatment by Chase ABC (20 mU/ml; 37°C; 40 min). The pretreatment with Chase ABC reduced PTN immunoreactivity. Scale bar, 25 μm. C, Western blotting analysis showing effects of CS-D and -A on PTN contents in cerebellar slice cultures. Addition of CS-D but not CS-A decreased the PTN contents in the slices.