Table 2.
Evaluation of the density of granule cells
| Culture conditions | Density of granule cells | |
|---|---|---|
| (Cells/150 μm IGL) | (Cells/100 × 100 μm2) | |
| Control | 189.6 ± 7.1 (28) | 95.5 ± 1.9 (28) |
| CS-D | 189.3 ± 8.9 (17) | 97.8 ± 2.6 (17) |
| Control IgG | 186.6 ± 6.1 (27) | 97.7 ± 2.2 (27) |
| α6B4PG | 189.0 ± 5.5 (25) | 91.7 ± 2.1 (25) |
Cerebellar slices derived from P9 rats were cultured for 6 d with or without CS-D (50 μg/ml), control IgG (200 μg/ml), and α6B4PG (200 μg/ml). Cryosections of slice cultures were stained by immunohistochemistry using an antibody against NeuN, and the NeuN-positive cells within 150 μm or 100 × 100 μm2 of the IGL were enumerated in confocal microscopic images of 2-μm-thick optical sections. Values are expressed as the mean ± SEM (n). Statistical analysis using Student'st test detected no significant difference between any set of conditions.