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The retinal whole mount doubly labeled for pChAT and a retrograde tracer, red latex FluoSperes, after injection into the contralateral superior colliculus. A, Confocal image of pChAT-immunoreactive cells in the retina (green).B, Retrogradely labeled cells with red latex FluoSperes (red). C, Superimposed image ofA and B showing double-labeled cells (yellow to orange).Arrows indicate corresponding cells. Scale bar, 50 μm.
