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. 2003 Apr 1;23(7):2872–2881. doi: 10.1523/JNEUROSCI.23-07-02872.2003

Fig. 5.

Fig. 5.

Expression of ChAT mRNA in the striatum and retina revealed by RT-PCR. A, Schematic drawing showing the exon organizations of cChAT and pChAT, as well as the positions of primers used. B, A 3% agarose gel showing the result of the first RT-PCR and the nested PCR for ChAT. Lane 1, Striatum (First PCR); lane 2, retina (First PCR); lane 3, striatum (Nest PCR); lane 4, retina (Nest PCR). The positions of DNA size markers (1 kb DNA ladder; Invitrogen) and those of positive bands are indicated to the left and right of the gel, respectively.