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Effects of darkness and light exposure on pChAT immunoreactivity in the primary visual pathway: the optic nerve head (A, D, G), optic chiasm (B, E, H), and optic tracts (C, F,I). A–C, Light exposure for 4 d. D–F, No treatment. G–I, Dark adaptation for 4 d. Arrows in C,F, and I indicate the optic tract. Note that both light exposure and dark adaptation are effective in increasing pChAT immunoreactivity in the visual pathway. Scale bar, 500 μm.
