Fig. 1.

Characterization of anti-EB3 antiserum. Novel anti-EB3 antibodies were raised in rabbit against GST-EB3 and characterized on Western blot. A, COS-1 cells were mock transfected or transfected with EB1, EB2, and EB3 fused to GFP. Thebottom panel (Western blot with anti-GFP antiserum) demonstrates that each fusion protein is expressed at a similar level. The top panel (Western blot with the novel anti-EB3 antiserum) shows that anti-EB3 antibodies recognize full-length EB3-GFP (marked by a double asterisk). EB1-GFP (marked by asingle asterisk) also is weakly recognized, whereas EB2-GFP is not. B, Equal amounts of whole-cell protein extract from HeLa, COS-1, and K562 cells and from mouse brain are analyzed. Two isoforms of EB3, which are expressed more abundantly in brain extracts, are recognized by the novel anti-EB3 antiserum.