Table 1.

Forward and backward movement of EB3-GFP in neurons

Compartment	Dashes			EB3-GFP displacements			Displacements per dash1-d
	na	% f1-b	% b1-c	n	% f	% b	f	b
	Hippocampal cultures
Proximal neurite	26	65	35	119	71	29	4.9	3.9
Distal neurite	15	87	13	55	87	12	3.7	3.5
Distal axon	9	100	0	37	100	0	4.1
Distal axon1-e	47	83	17	137	83	17	2.9	2.9
Growth cone	7	100	0	28	100	0	4
	PC cultures
Proximal dendrites	54	65	35	145	64	36	2.6	2.7
Distal dendrites	51	75	25	151	74	26	2.9	3
Axon 1	20	100	0	65	100	0	3.25
Axon 2	13	85	15	40	83	17	3	3.5

^F1-aNumber (n) of dashes observed.

^F1-bPercentage (%) of forward (f) moving dashes.

^F1-cPercentage (%) of backward (b) moving dashes.

^F1-dEB3-GFP displacements per dash were calculated by dividing the number of displacements by the number of dashes. With an average acquisition time of 2.5 sec/frame and average velocity of 0.22 μm/sec, the average displacement per dash is ∼1.65 μm.

^F1-eDistal axon near growth cone.