Fig. 3.

Scavenger receptors mediate Aβ stimulation of intracellular Tyr signaling cascades in THP-1 monocytes and microglia. THP-1 monocytes were preincubated with 300 μg/ml fucoidan (A) or 100 nm GST-CD36-(93–120) (B) for 30 min before stimulation with fibrillar or nonfibrillar Aβ. C, Primary murine microglia were treated with 100 nm GST-CD36-(93–120) before Aβ stimulation. Cell lysates were analyzed by Western blot using the anti-phospho-Tyr antibody 4G10 (PY) or an anti-phospho-ERK antibody (P-ERK). P-ERK blots were stripped and reprobed with an anti-ERK antibody (ERK) as a protein-loading control.