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. 2003 Jan 1;23(1):43–51. doi: 10.1523/JNEUROSCI.23-01-00043.2003

Fig. 2.

Fig. 2.

Immunoblot detection and quantitative analysis of Na,K-ATPase isoforms in nontransgenic controls (C, control), transgenic mutant SOD1 mice (M, mutant) and transgenic normal human SOD1 overexpressors (O, SOD++).A, B, Immunoblot and densitometric analysis of α1, α2, α3. For all immunoblots 50 μg of spinal cord homogenates was used. A, Separated protein was stained with monoclonal antibodies to α1 (6F), α2 (McB2), and α3 (XVIF9G10). Crude homogenates from rat brain served as positive controls for the antibodies (data not shown). Molecular weight markers are shown in kDa.B, Data are expressed as arbitrary units and represent the means ± SEM for an average of three experiments.C, Immunoblot stained with KETYY, an antibody that recognizes all Na,K-ATPase α subunits. D, Densitometric analysis of KETYY expressed in arbitrary units and representing the means ± SEM for an average of three experiments.E, Spinal cord homogenates were stained for β1 (SpETb1) and β2 (SpETb2) polyclonal antibodies. The data that are shown are representative of multiple experiments.