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. 2003 Jan 1;23(1):175–186. doi: 10.1523/JNEUROSCI.23-01-00175.2003

Fig. 7.

Fig. 7.

Domain 1 accounts for most of the inhibition caused by integral membrane NG2. A, Cerebellar explants from postnatal day 5–7 rats were grown for 40–44 hr on membrane carpets prepared from untransfected 293 cells, 293-NG2 cells, and 293-NG2 cell membranes treated with either polyclonal anti-NG2 antibodies or the anti-D1, anti-D2, and anti-D3N monoclonal antibodies. Data shown are mean ± SEM from at least three independent experiments. Between 16 and 34 individual explants were measured for each datapoint. *p < 0.001 versus HEK-293; Student's t test. B–G, Appearance of the explants on the different membrane substrates: B, 293 cells, C, 293-NG2 cells; D, 293-NG2 cells treated with rabbit anti-NG2; E, 293-NG2 cells treated with anti-D1; F, 293-NG2 cells treated with anti-D2; and G, 293-NG2 cells treated with anti-D3N. Scale bar, 200 μm.