Fig. 6.
a, Time course of changes in trkB autophosphorylation in response to fluoxetine administration (20 mg/kg, i.p.). b, Autophosphorylation of trkB after acute and chronic antidepressant treatments. Mice received saline (open bars) or imipramine (30 mg/kg) (filled bars) intraperitoneally once (30 min; n = 18 for both groups) or once daily for 3 weeks (21 d; n = 11 for both groups). Top panel, Representative Western blots precipitated with WGA and sequentially probed with anti-phosphotyrosine antibody 4G10 and anti-trkBout antibody. Bottom panel and a show quantitated levels of phosphorylated trkB from different treatments. Data are presented as means ± SEM percentages of the ratio of phosphorylated trk (4G10) to full-length trkB (anti-trkBout) signal intensity levels found in saline-treated animals. *p < 0.03, **p < 0.01 against saline control; Student'st test.