Fig. 1.

Smad1 and BMP4 protein expression are temporally regulated in the cerebellum. Protein levels increase during early postnatal ontogeny and decline in the adult. A, Representative immunoblot showing Smad1 expression in cerebellar tissue prepared from rats at the indicated postnatal and adult ages.B, Immunoblot of BMP4 expression in cerebellar tissues prepared at the indicated ages. Similar developmental profiles of Smad1 and BMP4 expression were observed in four independent experiments. This blot was stripped and reprobed for actin as a control for sample preparation and loading.