Fig. 6.

BMP4 induces granule neuron differentiation in cerebellar cultures. A, B, Images of MAP staining in cerebellar cells plated at P6 and grown for 3 d in the absence (A) or presence (B) of 10 ng/ml BMP4. BMP treatment promoted neuronal process outgrowth and branching in a dose-dependent manner. Similar changes were observed in cultures prepared at P10. C, Representative immunoblots of synaptotagmin 1a and MAP in cells maintained in culture in the absence or presence of 10 ng/ml BMP4 for 3 d. The BMP-induced changes in neuronal differentiation observed with immunofluorescence were accompanied by increases in the levels of these neuronal proteins.