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Presumed GABAergic neurons with low spontaneous firing rate are excited by orexins. A, Double stainings of biocytin-filled neuron (red) and TH-immunoreactive neurons (green). This neuron is TH negative.B, Voltage responses to current pulses (−0.2, 0,+0.1 pA). C, Chart recording of membrane potential and spontaneous action potentials before and after application of orexin A (100 nm).
